7. specimen emails. Specimen collection and preservation Oct. 17, 2012 65 likes 48,044 views Health & Medicine Its about various details of collection of specimen, techniques, presevation and processing. or false, Insert needle through chest wall into pleural. Proper sample collection leads to the proper diagnosis of disease. Diagnosis. Dr Ahmad Mohammad Ashshi (AIBMS) Consultant Virologist Head of Clinical Laboratory Medicine, Hera General Hospital On Honorary Teaching Panel, Faculty of Medicine, Umm-Al Qura University, Makkah. Template SPECIMEN Bronchoscopy specimen, bone marrow or CSF). the following procedures are to be followed for testing whole blood samples with the oxford, Specimen collection pgs. selection guidelines, refer to Table 4. foundation block (2018). Check the requisition form for requested tests, patient information, and any special requirements. Name and address of the hospital or health centre. 1. specimen for viral isolation specimens for the, Specimen Collection and Transport - . Its about various details of collection of specimen, techniques, presevation and processing. Source of specimen. jitendra kumar pandey,mgm medical college,mumbai. Midstream "Clean-Catch" Urine Specimen 4.3. describe avian. If so, share your PPT presentation slides online with PowerShow.com. Mysore University. Instant access to millions of ebooks, audiobooks, magazines, podcasts and more. COL E ION , H A single serum sample is required when testing for hepatitis B surface Antigen (HBsAg) and HIV-1 and HIV-2. Activate your 30 day free trialto unlock unlimited reading. (ii) For special cases (examination of endocrines and viral infection-like PPR) tissues are to be collected in Bouin's fluid. The most important aspects of microbiological testing are collection of the right specimen and transport of the specimen to lab in a manner that insure the reliability of the diagnostic procedure (e.g., culture, microscopy and antigen or antibody tests). Then you can share it with your target audience as well as PowerShow.coms millions of monthly visitors. sample collection. Laboratory Diagnostics, Specimen Collection, and Biosafety Issues. The two unique identifier will be The patients full name Medical record number, Patients Preparation Specimens collected during this "fasting" period will yield the most reliable results. Follow patient instructions prior to specimen collection Including the proper order of blood draw when multiple tubes are required. 0 Patients Preparation factors ,cont Diurnal variation: Diurnal rhythms are body fluid and analyte fluctuations during the day. test, Urine Collection Technician: Proficiency Demonstrations - Urine specimen collectors. The Phlebotomist/Courier practices safe driving . W\.\f0q[T`5Z)/L;:&2#d+38::,:::2: A"\45V7X9H500304,;1!hiSUf%98S p ;xD d;7 1-2 gm quantity is sufficient. Specimen Collection and Transport. Air drawn in from butterfly or connector tubing. PowerShow.com is brought to you byCrystalGraphics, the award-winning developer and market-leading publisher of rich-media enhancement products for presentations. <> May perform venipunctures, including blood culture collection, and fingersticks ensuring proper /labeling of specimens and using vein preservation techniques. Sample collection for bacterial isolation, characterization and antibiotic se Lecture 12-laboratory and field investigation, Collection and Handling of Specimens for Laboratory Diagnosis, Collection of biological specimens for microbiology tests, Collection, Preservation and Dispatch of Clinical Samples, U. P. 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Date and time of collection. - CrystalGraphics offers more PowerPoint templates than anyone else in the world, with over 4 million to choose from. xW-xq6QT;0)7,k*1 9dP\.Ats~kmZoL47uT?M:G| )if=K5. hmO9_6~?UUw:-'!^l*lP'c=cg~cJ5IDNhE 4zBCIda0 The recovery of Streptococcus pneumoniae, If it is a hospitalized patient, Pseudomonas, Non-bacterial organisms such as opportunistic, Viruses are not an infrequent cause of LRTs, Mycoplasma pneumoniae is the cause of primary, Legionellosis (caused by Legionella pneumophila), Mycobacteria, especially M. tuberculosis in the, Sheeps blood agar (SBA) is used for routine, S. pneumoniae, which is by far the most common, Chocolate agar is included because H. influenzae, MacConkey agar is included to isolate enteric, After assessing the quality of the sputum the, If there appears to be is a predominating, An experienced microbiologist often can make, Septicemia bacteria actively growing in the, Bacteremia bacteria being transported via the, Morbidity mortality occur in both cases, but, Occult bacteremia bacteremia with persistent, Intermittent bacteremia occurs when microbes are, Persistent bacteremia patient is constantly, Toxemia septicemia with persistent systemic, Blood sample contamination with skin microbes is, Apply 12 iodine antiseptic, allow to dry 1-2, Taking multiple blood samples increases the, Ideally a blood culture should be taken just as, If this is not possible (not all patients with, If antibiotics need to be given immediately, two, The volume of blood collected is the most, The minimum volume of blood for a single adult, 20 to 30 mL per sample is advocated by many, For each 1mL increase of blood/culture, there, Blood contains many natural antimicrobial, Additionally, when the blood is collected, In order to negate these antimicrobials blood, Common blood culture media are liquid and contain, The ideal blood to media ratio (see above) is 1, Many commercially automated blood culture systems, Use of anticoagulants is essential to prevent, Anticoagulants used must not inhibit the growth, In addition to anticoagulant properties, SPS, SPS interferes with the antibacterial action of, SPS may inhibit the growth of certain pathogenic, A blood sample is distributed between two (or, One bottle of media, best suited for growing, The other bottle is not vented (?CO2 ?O2) and, Macroscopic examination bottles are observed for, An aliquot of a well-mixed broth and blood is, Blind subculture of aerobic bottles showing no, Chocolate agar is the best medium for routine, Chocolate agar subcultures are incubated under, Advantages of a conventional broth-based-non-autom, The whole process is labor intensive because, Results of positive conventional blood cultures, Blood samples are processed as usual, inoculated, Once placed in the incubator/monitor/computer, Monitoring is continuous (usually once every ten, Time course data - growth curve - kinetics, Visual and/or audible results signals are given, Routine blind subcultures are not necessary, Incubation times are usually shorter than for, Optical density meter to detect turbidity, CO2 evolution (or consumption?) transportation of specimens, and consider how When to Request Transport . Never give the specimen to someone else to label. Uses of laboratory test To provide information relating to a clinical diagnosis To investigate the etiology of a clinical condition To study complications of a disease or its treatment To provide prognostic information To monitor natural history or response to Rx. WHAT IS THE MOST IMPORTANT THING YOU NEED FOR SPECIMEN COLLECTION??? Title: Microsoft PowerPoint - sample collection [Compatibility Mode] Author: Nishi Created Date: 5/25/2017 6:51:58 PM . role of the nurse. Patients should be informed that urine and some of the blood specimens are generally collected during the early morning hours after waking and before eating, usually about 8 to 12 hours after the last ingestion of food or drink, Patients Preparation,cont. prior to antibiotics or dosing schedules). Mix specimen thoroughly, then insert a sterile, Place the filled loop flat onto the agar and drag, Streak perpendicular to the 1 streak in a way, Plating 0.01mL of urine is equivalent to making a, For statistical validity, the number of colonies, The reporting unit is CFU/mL and not colonies, Making serial dilutions of urine in sterile water, Even though this method is very accurate it is, The kind of accuracy this method allows is not, Properly collected catheterized urine rarely is, However, the process of catheterizing is a risk, If the patient is going to be catheterized for, Urine taken from an indwelling catheter must not, The specimen should be taken aseptically with a, Again, this is not recommended for routine, It is now known that clean-catch counts are, The urethral opening is colonized with a variety, Collection of mid-stream urine prevents much of, Clean-catch applies to urine that does not make, This is easy for males, but often requires, These samples are best taken first thing in the, Urine specimens must be processed within 2 hrs of, Suprapubic aspiration (SPA), another method of, Suprapubic aspirated urine is the only type of, Suprapubic aspiration is done by qualified, Then a needle is inserted through the skin and a, Cystoscopy is a procedure that uses a fiber-optic, During this exam urine specimens can be collected, Properly collected, freshly voided urine from a, A given UTI is usually caused by a single species, If gt 3 organisms grow in a urine specimen (other, If 2 different organisms grow at a colony count, Direct microscopic examination of urine for, Occasionally a physician will request a Gram, A drop of unspun urine is placed on a microscope, If the number of microbial cells averages one or, Meningitis is inflammation of the meninges. 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